Senior Scientist, Molecular Biology & Cell Engineering at Apex Systems in Waltham, MA

Apex Systems

๐Ÿ“Œ Waltham, MA
๐Ÿ•‘ November 23, 2020
๐Ÿท๏ธ OTHER
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Senior Scientist, Molecular Biology and Cell Engineering

Scientist/Sr Scientist, Molecular Biology and Cell Engineering 1091050

Job Scope & Purpose:

Our exclusive clientis seeking a highly motivated and experienced senior/principal research associate/scientist with a strong focus on molecular biology, mammalian cell culture, viral transduction, and flow cytometry to join the Biologics team. The successful candidate will be a key member of the antibody discovery group responsible for design of plasmids and cloning, viral transduction and cell line development, CRISPR-mediated gene manipulation, and flow cytometric evaluation.

Primary

Job Responsibilities

  • Vector Design and cloning of plasmids for expression of recombinant proteins, antibodies and mutli-specificAbs
  • Virus-mediated transduction and creation of isogenic cell lines expressing surface proteins
  • Cell-based screening and Flow cytometric evaluation and characterization of antibodies/bi-specifics
  • CRISPR/Cas9 mediated manipulation of cell lines for gene knock-in or knock-out
  • Molecular biology including sequence analysis, qPCR, RT-PCR, restriction digest and gel electrophoresis
  • Mammalian cell culture and high throughput protein production
  • Interface with CROs for sequence analysis, DN
    A scale-up, and cell line development
  • Generate, manage, analyze, and maintain critical data in a highly organized manner
  • Primary

    Job Requirements

  • Ph. D. with 0-6 years of industry experience in cell line development and characterization (title and salary commensurate with experience)
  • Extensive experience in vector design, Gibson, Golden Gate and restriction cloning, and creation of plasmids for expression of recombinant proteins and antibodies/Bi-specifics
  • Proficiency in design and creation of retroviral/lentiviral plasmids, transduction of cell lines and primary cells
  • Experience in NGS and related data analysis is a plus
  • Hands-on experience in CRISPR/Cas9 mediated gene manipulation
  • Proficient in Flow Cytometric evaluation and characterization of cell lines for antibody binding and potency
  • Experience in extraction of immune cells from blood and their vitro characterization will be preferred
  • Experience in protein expression using Expi293 and ExpiCHO cells is a plus
  • Excellent aseptic skills, and mammalian cell culture experience is a must
  • Hands-on experience with standard molecular biology skills including bacterial culture and transformation, qPCR, RT-PCR, DNA purification, restriction digestion, sequence analysis and vector construction
  • Experience working with CROs for sequence analysis, DN
    A scale-up and cell line development
  • Efficient time management and ability to work on several projects simultaneously
  • Strong interpersonal skills and ability to effectively communicate with members of the multidisciplinary team
  • The ability to work collaboratively, provide strategic input,communicate clearly both written and orally is critical for success in this position.
  • Excellent record keeping abilities, to document analytical data generated in support of regulatory requirements, adherence to GDP, experience with ELN
  • About Stratacuity:

    Whether you are seeking a career change or simply interested in becoming part of our network, you will appreciate the ethics guiding each Stratacuity team member. We build lasting relationships with exceptional biopharmaceutical talent and take great care to protect your personal information. Upon receiving your inquiry, you may be directly contacted by a Stratacuity team member to discuss your career goals. We will not share your information with anyone without your direct prior consent.
    PROVEN SCIENTIFIC PLACEMENT

    Stratacuity is an Equal Employment Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Stratacuity will consider qualified applicants with criminal histories in a manner consistent with the requirements of applicable law. If you have visited our website in search of information on employment opportunities or to apply for a position, and you require an accommodation in using our website for a search or application, please contact us.

    Code: DiscDevt

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    Job Expires: December 23, 2020

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